6KFN
Crystal structure of alginate lyase from Paenibacillus sp. str. FPU-7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-05-13 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.85 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 125.635, 57.764, 39.714 |
| Unit cell angles | 90.00, 91.10, 90.00 |
Refinement procedure
| Resolution | 23.357 - 0.890 |
| R-factor | 0.106 |
| Rwork | 0.105 |
| R-free | 0.12160 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 0.910 |
| High resolution limit [Å] | 0.890 | 0.890 |
| Rmerge | 0.050 | 0.672 |
| Number of reflections | 200667 | 9592 |
| <I/σ(I)> | 29.5 | 2.6 |
| Completeness [%] | 92.6 | 88.5 |
| Redundancy | 4.4 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | PEG8000, imidazole |
