6KEX

Crystal structure of reduced phosphoribulokinase from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	P 1
Unit cell lengths	49.835, 80.359, 103.087
Unit cell angles	70.10, 82.26, 89.85

Refinement procedure

Resolution	49.330 - 2.500
Rwork	0.211
R-free	0.25630
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.330	2.589
High resolution limit [Å]	2.500	2.500
Rmerge	0.146	0.763
Number of reflections	50674	2506
<I/σ(I)>	10.9
Completeness [%]	95.3
Redundancy	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	4% v/v Tacsimate pH5.0, 12% w/v polyethylene glycol 3350