6KEW
Crystal structure of oxidized phosphoribulokinase from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-04-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 80.784, 50.315, 194.783 |
| Unit cell angles | 90.00, 91.81, 90.00 |
Refinement procedure
| Resolution | 42.700 - 2.290 |
| Rwork | 0.241 |
| R-free | 0.25930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6kex |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.330 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.085 | 0.553 |
| Rmeas | 0.092 | 0.601 |
| Rpim | 0.036 | 0.232 |
| Number of reflections | 23859 | 1703 |
| <I/σ(I)> | 18.1 | 2.22 |
| Completeness [%] | 95.1 | 98.9 |
| Redundancy | 6.3 | 6.5 |
| CC(1/2) | 0.978 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 4% v/v Tacsimate pH5.0, 12% w/v polyethylene glycol 3350 |
