6KEN
Crystal structure of Drosophila melanogaster Noppera-bo, glutathione S-transferase epsilon 14 (DmGSTE14), in glutathione-bound form
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2016-02-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.748, 75.674, 107.839 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.700 - 1.750 |
| R-factor | 0.1742 |
| Rwork | 0.173 |
| R-free | 0.20690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6kem |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.888 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.10.1_2155, 1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.700 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.082 | 1.052 |
| Rmeas | 0.085 | |
| Number of reflections | 49238 | 4699 |
| <I/σ(I)> | 14.3 | |
| Completeness [%] | 100.0 | 97.3 |
| Redundancy | 14.3 | 12.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293 | 42.5% (v/v) PPG400, 100 mM Bis-Tris, and pH 6.4 |
