6KEA

crystal structure of MBP-tagged REV7-IpaB complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-20
Detector	PSI PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	91.829, 146.707, 102.468
Unit cell angles	90.00, 116.58, 90.00

Refinement procedure

Resolution	29.910 - 2.350
Rwork	0.192
R-free	0.22630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3vd8 3abd
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14-3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.910	2.430
High resolution limit [Å]	2.350	2.350
Rmerge	0.137	0.873
Number of reflections	98435
<I/σ(I)>	14.22
Completeness [%]	97.5	97.8
Redundancy	7.2	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	100 mM Bistris, pH 5.6, 25% PEG3350