6KE9

The Human Telomeric Nucleosome Displays Distinct Structural and Dynamic Properties

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-03
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	106.133, 109.319, 176.969
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.010 - 2.220
R-factor	0.205
Rwork	0.204
R-free	0.23000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lz0
RMSD bond length	0.010
RMSD bond angle	0.940
Data scaling software	autoPROC
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	93.000	2.220
High resolution limit [Å]	2.180	2.180
Rmerge	0.050	1.230
Rpim	0.012	0.343
Number of reflections	53570	5306
<I/σ(I)>	26.1	2.1
Completeness [%]	99.3
Redundancy	13.2
CC(1/2)	1.000	0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	Manganase chloride, potassium chloride, potassium cacodylate, MPD and trehalose