6KE4

ABloop reengineered Ferritin Nanocage

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.99779
Spacegroup name	F 4 3 2
Unit cell lengths	182.642, 182.642, 182.642
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.440 - 2.300
R-factor	0.1948
Rwork	0.190
R-free	0.23580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fha
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.080	0.424
Rmeas		0.424
Number of reflections	12159	533
<I/σ(I)>	13.62	2.37
Completeness [%]	99.9	88.8
Redundancy	5.7	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.1M Bincine pH 9.0, 2M MgCl2