6KDV

Crystal structure of TtCas1-DNA complex

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-05-19
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97910,0.97937,0.96414
Spacegroup name	P 21 21 2
Unit cell lengths	116.547, 201.199, 77.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.890 - 3.110
R-factor	0.2413
Rwork	0.239
R-free	0.27920
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	0.954
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.150
High resolution limit [Å]	3.100	3.100
Number of reflections	33449	1633
<I/σ(I)>	19
Completeness [%]	99.6
Redundancy	18.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.2M ammonium chloride, 0.1M calcium chloride, 20% PEG 4000, 0.05M Tris-HCl