6KD7
Crystal structure of geranylgeranyl pyrophosphate synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-12-05 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97934 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 194.718, 194.718, 107.544 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.430 - 2.000 |
R-factor | 0.227 |
Rwork | 0.222 |
R-free | 0.24650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5jfq |
RMSD bond length | 0.014 |
RMSD bond angle | 1.836 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.086 | 0.298 |
Number of reflections | 78959 | 3761 |
<I/σ(I)> | 28.1 | 3.3 |
Completeness [%] | 97.5 | 94.8 |
Redundancy | 10.8 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | Ammonium sulfate, HEPES |