6KD3
Structural and catalytic analysis of two diverse uridine phosphorylases in the oomycete Phytophthora capsici.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-03 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.836, 66.386, 261.061 |
| Unit cell angles | 90.00, 96.77, 90.00 |
Refinement procedure
| Resolution | 47.450 - 2.764 |
| R-factor | 0.3877 |
| Rwork | 0.386 |
| R-free | 0.42780 |
| Structure solution method | MAD |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.860 |
| High resolution limit [Å] | 2.760 | 2.760 |
| Rmerge | 0.235 | |
| Number of reflections | 31606 | 1242 |
| <I/σ(I)> | 6.39 | 1.7 |
| Completeness [%] | 82.0 | 84.4 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 283 | 0.2M Ammonium iodide, 20% PEG 3350 |
