6KD1

Room temperature structure of lysozyme delivered in agarose by serial millisecond crystallography

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	298.15
Detector technology	PIXEL
Collection date	2019-04-03
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.9796
Spacegroup name	P 43 21 2
Unit cell lengths	79.740, 79.740, 38.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.385 - 1.850
R-factor	0.1762
Rwork	0.173
R-free	0.22440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ig6
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.600	1.910
High resolution limit [Å]	1.850	1.850
Number of reflections	11117	1068
<I/σ(I)>	6.33
Completeness [%]	100.0	100
Redundancy	361.4	250.4
CC(1/2)	0.995	0.823

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES		293.5	sodium acetate, PEG 8000, NaCl