6KCW
Structure of alginate lyase Aly36B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-05-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9791 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 53.770, 89.492, 46.856 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.860 - 2.280 |
R-factor | 0.197 |
Rwork | 0.195 |
R-free | 0.23500 |
Structure solution method | SAD |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.860 | 2.380 |
High resolution limit [Å] | 2.280 | 2.280 |
Rmerge | 0.137 | |
Number of reflections | 10564 | 1026 |
<I/σ(I)> | 25.06 | |
Completeness [%] | 98.8 | |
Redundancy | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | potassium phosphate monobasic/ sodium phosphate dibasic, polyethylene glycol (PEG) 8,000, and sodium chloride. |