6KCF

Structure of Inosine 5'-monophosphate Dehydrogenase from Candidatus Liberibacter asiaticus str. psy62

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2018-06-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	143.126, 134.857, 85.617
Unit cell angles	90.00, 101.21, 90.00

Refinement procedure

Resolution	42.469 - 2.550
R-factor	0.2244
Rwork	0.222
R-free	0.26450
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.469	3.000
High resolution limit [Å]	2.550	2.550
Number of reflections	51023	50928
<I/σ(I)>	19.9
Completeness [%]	98.2
Redundancy	2.58

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	30% PEG 400, 100 mM HEPES pH 7.5, 200 mM NaCl