6KCD

Room temperature structure of lysozyme delivered in shortening B by serial millisecond crystallography

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	293.15
Detector technology	PIXEL
Collection date	2019-04-03
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.9796
Spacegroup name	P 43 21 2
Unit cell lengths	79.450, 79.450, 38.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.989 - 1.500
R-factor	0.1862
Rwork	0.185
R-free	0.20690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ig6
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.000	1.550
High resolution limit [Å]	1.500	1.500
Number of reflections	20406	1995
<I/σ(I)>	9.27	1.68
Completeness [%]	100.0	100
Redundancy	756.6	285.8
CC(1/2)	0.994	0.518

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	4.5	293.5	sodium acetate, PEG 8000, NaCl