6KCC

Room temperature structure of glucose isomerase delivered in shortening B by serial millisecond crystallography

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	293.15
Detector technology	PIXEL
Collection date	2019-04-03
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.9796
Spacegroup name	I 2 2 2
Unit cell lengths	94.190, 99.920, 103.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.803 - 2.000
R-factor	0.176
Rwork	0.176
R-free	0.20100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zyd
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.460	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	33248	3247
<I/σ(I)>	4.1
Completeness [%]	100.0	100
Redundancy	701.6	481.7
CC(1/2)	0.967	0.578

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	7	293.5	Tris-HCl, Ammonium sulfate, Magnesium sulfate