6KC0
fused To-MtbCsm1 with 2ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.628, 53.622, 130.313 |
| Unit cell angles | 90.00, 98.97, 90.00 |
Refinement procedure
| Resolution | 64.359 - 2.295 |
| R-factor | 0.2176 |
| Rwork | 0.213 |
| R-free | 0.28570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4uw2 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.368 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.14_3247: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.539 | 2.377 |
| High resolution limit [Å] | 2.295 | 2.295 |
| Rmeas | 0.095 | |
| Rpim | 0.053 | 0.343 |
| Number of reflections | 34833 | 3276 |
| <I/σ(I)> | 12.31 | |
| Completeness [%] | 96.6 | |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M BICINE pH 8.7 and 15% PEG 1,500 |
