6KBS
Crystal structure of yedK in complex with ssDNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-01-08 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.890, 50.322, 54.531 |
| Unit cell angles | 90.00, 99.08, 90.00 |
Refinement procedure
| Resolution | 37.757 - 1.601 |
| R-factor | 0.1742 |
| Rwork | 0.173 |
| R-free | 0.20080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6kbu |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.845 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 3.450 | 1.600 |
| Rmerge | 0.060 | 0.033 | 0.602 |
| Rmeas | 0.065 | 0.035 | 0.659 |
| Rpim | 0.025 | 0.014 | 0.262 |
| Total number of observations | 214311 | ||
| Number of reflections | 32253 | 3292 | 3202 |
| <I/σ(I)> | 4.8 | ||
| Completeness [%] | 99.4 | 98.8 | 99.6 |
| Redundancy | 6.6 | 6.7 | 5.9 |
| CC(1/2) | 0.999 | 0.862 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | 0.1 M Citric pH 4.0, 3% PEG6000 |
