6KBS

Crystal structure of yedK in complex with ssDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-08
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	91.890, 50.322, 54.531
Unit cell angles	90.00, 99.08, 90.00

Refinement procedure

Resolution	37.757 - 1.601
R-factor	0.1742
Rwork	0.173
R-free	0.20080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6kbu
RMSD bond length	0.007
RMSD bond angle	0.845
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	1.660
High resolution limit [Å]	1.600	3.450	1.600
Rmerge	0.060	0.033	0.602
Rmeas	0.065	0.035	0.659
Rpim	0.025	0.014	0.262
Total number of observations	214311
Number of reflections	32253	3292	3202
<I/σ(I)>	4.8
Completeness [%]	99.4	98.8	99.6
Redundancy	6.6	6.7	5.9
CC(1/2)		0.999	0.862

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	0.1 M Citric pH 4.0, 3% PEG6000