6K9Z
STRUCTURE OF URIDYLYLTRANSFERASE MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-06-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 62.188, 62.188, 308.594 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.760 - 1.780 |
| R-factor | 0.1741 |
| Rwork | 0.173 |
| R-free | 0.19560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6k5z |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.911 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.810 |
| High resolution limit [Å] | 1.780 | 4.830 | 1.780 |
| Rmerge | 0.064 | 0.036 | 0.540 |
| Rmeas | 0.069 | 0.038 | 0.583 |
| Rpim | 0.025 | 0.013 | 0.214 |
| Total number of observations | 465036 | ||
| Number of reflections | 68099 | 3771 | 3317 |
| <I/σ(I)> | 6.8 | ||
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 6.8 | 8.8 | 6.9 |
| CC(1/2) | 0.999 | 0.855 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | PEG 600, calcium acetate, cacodylate buffer, UDP-Glu |
