6K9J
0.98 A three-dimensional structure of horse heart cytochrome C at 110K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2016-05-14 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 43 |
| Unit cell lengths | 56.887, 56.887, 42.487 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 0.980 |
| Rwork | 0.131 |
| R-free | 0.15260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hrc |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | MOLREP |
| Refinement software | SHELXL (2017/1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.400 | 1.010 |
| High resolution limit [Å] | 0.980 | 0.980 |
| Rmerge | 0.062 | 0.301 |
| Number of reflections | 77173 | 7715 |
| <I/σ(I)> | 26.02 | 3.43 |
| Completeness [%] | 99.1 | 100 |
| Redundancy | 5.2 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 293 | 1.0-1.5M sodium chloride, 2.7-3.2M ammonium sulfate, 0.1M sodium phosphate |
