6K9E

The A form apo structure of NrS-1 C terminal region-CTR(305-718)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-10-17
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	159.692, 159.906, 159.709
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.689 - 2.900
R-factor	0.2379
Rwork	0.236
R-free	0.26430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.846
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.130	0.485
Rmeas	0.191	0.546
Number of reflections	92216	9024
<I/σ(I)>	8.8	2.2
Completeness [%]	99.7	98.8
Redundancy	8.2	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291.15	1.0M sodium citrate pH 7.0, 0.2M Nacl