6K9C
The apo structure of NrS-1 C terminal region (305-718)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-01-19 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 161.130, 161.130, 161.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.921 - 2.406 |
| R-factor | 0.1952 |
| Rwork | 0.193 |
| R-free | 0.24080 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.846 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL2Map |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.087 | 0.492 |
| Rmeas | 0.090 | 0.545 |
| Number of reflections | 54069 | 2622 |
| <I/σ(I)> | 21.3 | 2.4 |
| Completeness [%] | 99.7 | 98.1 |
| Redundancy | 12.5 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 291.15 | 1.6 M NaH2PO4/0.4 M K2HPO4, 0.1 M phosphate-citrate pH 4.2 |
