6K96
Crystal structure of Ari2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-09 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 101.180, 101.180, 389.662 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.180 - 2.500 |
| R-factor | 0.229 |
| Rwork | 0.226 |
| R-free | 0.28040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gr0 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.928 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.138 | 0.407 |
| Number of reflections | 40934 | 5751 |
| <I/σ(I)> | 12.4 | 4.6 |
| Completeness [%] | 97.4 | 96.3 |
| Redundancy | 8.4 | 8.7 |
| CC(1/2) | 0.990 | 0.867 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 278 | Tris-HCl, sodium chloride, sodium citrate, NAD |
