6K91
Pyridoxal Kinase from Leishmania donovani in complex with ADP and Pyridoxine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2018-05-17 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.700, 71.690, 77.140 |
| Unit cell angles | 90.00, 99.28, 90.00 |
Refinement procedure
| Resolution | 43.120 - 2.000 |
| R-factor | 0.1758 |
| Rwork | 0.174 |
| R-free | 0.21785 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.553 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.120 | 2.040 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 37788 | 2712 |
| <I/σ(I)> | 10.1 | |
| Completeness [%] | 95.0 | 91.9 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 17% PEG 8000, 0.1M Sodium Cacodylate, 0.32M Calcium Acetate, 6% Glycerol |
