6K8P
Structural and catalytic analysis of two diverse uridine phosphorylases in the oomycete Phytophthora capsici.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-01-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 66.956, 97.330, 188.346 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.463 - 1.965 |
| R-factor | 0.2675 |
| Rwork | 0.265 |
| R-free | 0.31560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6k5g |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.199 | 0.984 |
| Number of reflections | 87357 | 8200 |
| <I/σ(I)> | 11.9 | |
| Completeness [%] | 99.7 | |
| Redundancy | 12.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 283 | PEG 4000, potassium phosphate, magenesium chloride, cacodylate |
