6K8I

Crystal structure of Arabidopsis thaliana CRY2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	C 2 2 21
Unit cell lengths	123.125, 160.372, 139.918
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.035 - 2.697
R-factor	0.188
Rwork	0.185
R-free	0.23680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.068
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.820
High resolution limit [Å]	2.697	2.697
Rmerge	0.084	0.763
Rmeas	0.090	0.824
Rpim	0.033	0.308
Number of reflections	38415	4594
<I/σ(I)>	16.4	2.6
Completeness [%]	99.9	99.2
Redundancy	7.4	7
CC(1/2)	0.999	0.862

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	MES, Potassium chloride, Pentaerythritol propoxylate (5/4 PO/OH).