6K85

The closed state of RGLG1 mutant-D338A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AichiSR BEAMLINE BL2S1
Synchrotron site	AichiSR
Beamline	BL2S1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-09-21
Detector	ADSC QUANTUM 315
Wavelength(s)	0.978897
Spacegroup name	P 21 21 21
Unit cell lengths	45.692, 72.871, 79.444
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.722 - 1.606
R-factor	0.1964
Rwork	0.195
R-free	0.22390
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.067
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.630
High resolution limit [Å]	1.600	4.340	1.600
Rmerge	0.173	0.125	1.175
Rmeas	0.181	0.130	1.281
Rpim	0.051	0.038	0.487
Total number of observations	384878
Number of reflections	33665	1924	1133
<I/σ(I)>	2.8
Completeness [%]	96.2	99.9	66
Redundancy	11.4	11.9	5.1
CC(1/2)		0.992	0.300

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.1 M MgCl2, 0.1M Bis-Tris ph 5.5, 25%PEG3350