6K7F
Crystal structure of MBPholo-Tim21 fusion protein with a 17-residue helical linker
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-02 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.970, 69.410, 171.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.282 - 1.800 |
| R-factor | 0.1766 |
| Rwork | 0.174 |
| R-free | 0.21630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1anf 2ciu |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.317 |
| Data reduction software | XDS (Jan 26, 2018) |
| Data scaling software | XDS (Jan 26, 2018) |
| Phasing software | PHENIX (1.8_1069) |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.047 | |
| Number of reflections | 46285 | 7366 |
| <I/σ(I)> | 19.26 | 2.02 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 6.6 | |
| CC(1/2) | 0.885 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M K/Na Tartrate, 16% PEG 3350 (w/v), microseeds |
