6K7F

Crystal structure of MBPholo-Tim21 fusion protein with a 17-residue helical linker

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9000
Spacegroup name	P 21 21 21
Unit cell lengths	40.970, 69.410, 171.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.282 - 1.800
R-factor	0.1766
Rwork	0.174
R-free	0.21630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1anf 2ciu
RMSD bond length	0.010
RMSD bond angle	1.317
Data reduction software	XDS (Jan 26, 2018)
Data scaling software	XDS (Jan 26, 2018)
Phasing software	PHENIX (1.8_1069)
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.910
High resolution limit [Å]	1.800	1.800
Rmerge	0.047
Number of reflections	46285	7366
<I/σ(I)>	19.26	2.02
Completeness [%]	99.9	99.8
Redundancy	6.6
CC(1/2)		0.885

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M K/Na Tartrate, 16% PEG 3350 (w/v), microseeds