6K74

Crystal structure of AMPPNP bound Ck1a alpha from C. neoformans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-27
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.97
Spacegroup name	C 2 2 21
Unit cell lengths	89.283, 95.853, 93.396
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.927 - 2.280
R-factor	0.2361
Rwork	0.230
R-free	0.28780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lp4
RMSD bond length	0.009
RMSD bond angle	1.110
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.930	2.362
High resolution limit [Å]	2.280	2.280
Number of reflections	18629	1797
<I/σ(I)>	8.82
Completeness [%]	99.5	98.03
Redundancy	13.6
CC(1/2)	0.997	0.838

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	290	0.2M lithium sulfate, 0.1M Tris pH 8.5, 30%(w/v) PEG 4000, 30% dextran sulfate sodium salt, 5mM AMPPNP, 5mM MgCl2