6K6N
Crystal structure of SIVmac239 Nef protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-31 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 72.531, 72.531, 111.087 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.266 - 2.000 |
| R-factor | 0.197435856953 |
| Rwork | 0.195 |
| R-free | 0.24053 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1avv |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.553 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 41.610 | 41.610 | 2.050 |
| High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
| Rmerge | 0.112 | 0.019 | 1.035 |
| Rmeas | 0.115 | 0.019 | 1.063 |
| Rpim | 0.026 | 0.005 | 0.242 |
| Total number of observations | 229843 | 2504 | 16705 |
| Number of reflections | 12304 | 188 | 884 |
| <I/σ(I)> | 20 | 54.6 | 3.2 |
| Completeness [%] | 100.0 | 99.3 | 100 |
| Redundancy | 18.7 | 13.3 | 18.9 |
| CC(1/2) | 0.999 | 1.000 | 0.874 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.1M Sodium citrate, 0.2M Ammonium acetate, 30% PEG4000 |
