6K6M
Crystal structure of HIV-2 Nef protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.577, 44.770, 103.636 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.170 - 2.072 |
| R-factor | 0.2063 |
| Rwork | 0.205 |
| R-free | 0.23980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1avv |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.555 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.27) |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 44.770 | 44.770 | 2.130 |
| High resolution limit [Å] | 2.070 | 9.030 | 2.070 |
| Rmerge | 0.055 | 0.013 | 0.779 |
| Rmeas | 0.059 | 0.014 | 0.840 |
| Rpim | 0.022 | 0.006 | 0.310 |
| Total number of observations | 91696 | 1077 | 6952 |
| Number of reflections | 12922 | 192 | 970 |
| <I/σ(I)> | 24.1 | 73.1 | 2.6 |
| Completeness [%] | 99.9 | 99.1 | 99.5 |
| Redundancy | 7.1 | 5.6 | 7.2 |
| CC(1/2) | 1.000 | 1.000 | 0.799 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.1M MES, 10% PEG 3350 |
