6K6L
YGL082W-catalytic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-12-18 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.940, 70.640, 68.380 |
| Unit cell angles | 90.00, 102.54, 90.00 |
Refinement procedure
| Resolution | 66.750 - 1.770 |
| R-factor | 0.19175 |
| Rwork | 0.189 |
| R-free | 0.24111 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jkn |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.145 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.750 | 1.833 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Number of reflections | 54216 | 54191 |
| <I/σ(I)> | 13.9 | |
| Completeness [%] | 98.0 | |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6.5 | 291 | 0.2 M Calcium acetate hydrate, 0.1 M Sodium cacodylate trihydrate pH 6.5, 18% w/v Polyethylene glycol 8000 |
