6K6K
The crystal structure of light-driven cyanobacterial chloride importer (N63A/P118A) Mastigocladopsis repens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2018-11-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 60.972, 60.972, 205.574 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.829 - 2.197 |
| R-factor | 0.2144 |
| Rwork | 0.212 |
| R-free | 0.26840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5itc |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.518 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.830 | 2.240 |
| High resolution limit [Å] | 2.197 | 2.200 |
| Number of reflections | 11542 | 489 |
| <I/σ(I)> | 17.1 | |
| Completeness [%] | 93.0 | |
| Redundancy | 12.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 4.9 | 293 | Magnesium nitrate hexahydrate, Sodium citrate |
