6K6J

The crystal structure of light-driven cyanobacterial chloride importer from Mastigocladopsis repens with Bromide ion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	93
Detector technology	CCD
Collection date	2017-09-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.987
Spacegroup name	P 63 2 2
Unit cell lengths	61.618, 61.618, 240.176
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.800 - 2.500
R-factor	0.197
Rwork	0.194
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5itc
RMSD bond length	0.010
RMSD bond angle	1.000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.800	2.560
High resolution limit [Å]	2.500	2.500
Number of reflections	8644	517
<I/σ(I)>	10.6
Completeness [%]	85.0
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	4.9	293	Magnesium nitrate hexahydrate, Sodium citrate