6K6A
Application of anti-helix antibodies in protein structure determination (8188cys-3LRHcys)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2016-07-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.227, 40.603, 61.691 |
| Unit cell angles | 87.16, 75.20, 65.50 |
Refinement procedure
| Resolution | 25.990 - 1.940 |
| R-factor | 0.17366 |
| Rwork | 0.169 |
| R-free | 0.21525 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6k64 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.558 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rpim | 0.035 | 0.091 |
| Number of reflections | 21978 | 1985 |
| <I/σ(I)> | 27.2 | |
| Completeness [%] | 94.8 | |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 296 | 45% PEG 4000, 0.1M HEPES pH 7.5 |
