6K69

Application of anti-helix antibodies in protein structure determination (9213-3LRH)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	103
Detector technology	CCD
Collection date	2016-09-23
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 32 2 1
Unit cell lengths	94.305, 94.305, 101.906
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.230 - 2.401
R-factor	0.2073
Rwork	0.204
R-free	0.23440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lrh 2huk
RMSD bond length	0.005
RMSD bond angle	0.674
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.500
High resolution limit [Å]	2.400	2.400
Rpim	0.024	0.203
Number of reflections	20907	2071
<I/σ(I)>	19
Completeness [%]	100.0
Redundancy	9.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		296	4.8M Sodium formate, 0.1M Sodium acetate pH 4.5