6K67
Application of anti-helix antibodies in protein structure determination (9011-3LRH)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2016-07-19 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | H 3 |
Unit cell lengths | 115.298, 115.298, 93.275 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.380 - 1.950 |
R-factor | 0.20441 |
Rwork | 0.203 |
R-free | 0.23650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lrh 2w73 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.505 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rpim | 0.043 | 0.260 |
Number of reflections | 33481 | 3373 |
<I/σ(I)> | 27.3 | |
Completeness [%] | 99.7 | |
Redundancy | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 296 | 50% PEG 400, 0.2M CaCl2, 0.1M HEPES pH 7.5 |