6K5Z

Structure of uridylyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 43
Unit cell lengths	73.194, 73.194, 126.036
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.330
R-factor	0.1956
Rwork	0.192
R-free	0.25490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gup
RMSD bond length	0.014
RMSD bond angle	1.659
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.370
High resolution limit [Å]	2.330	6.320	2.330
Rmerge	0.081	0.032	0.761
Rmeas	0.087	0.035	0.840
Rpim	0.032	0.013	0.349
Total number of observations	209601
Number of reflections	28397	1464	1418
<I/σ(I)>	11.6
Completeness [%]	100.0	99.7	99.4
Redundancy	7.4	7.1	5.6
CC(1/2)		0.999	0.785

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	PEG 8000, Mg acetate, MES buffer