6K5X

Crystal Structure of the DNA-Binding Domain of GapR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	I 41 2 2
Unit cell lengths	47.422, 47.422, 225.502
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.532 - 2.086
R-factor	0.251
Rwork	0.251
R-free	0.25850
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.742
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.720	2.120
High resolution limit [Å]	2.080	2.080
Rmerge	0.100	0.530
Number of reflections	8095	363
<I/σ(I)>	48.8
Completeness [%]	98.1	95
Redundancy	22.2	12.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	15% PEG4000, 0.1M sodium cacodylate,0.001M GGH, 0.001GSSG, PH6.0