6K5J
Structure of a glycoside hydrolase family 3 beta-N-acetylglucosaminidase from Paenibacillus sp. str. FPU-7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 84.230, 84.230, 157.805 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.616 - 1.903 |
| R-factor | 0.1636 |
| Rwork | 0.162 |
| R-free | 0.20390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bmx |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.500 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.101 | 0.967 |
| Number of reflections | 45405 | 2994 |
| <I/σ(I)> | 19.4 | 2.9 |
| Completeness [%] | 100.0 | 99.5 |
| Redundancy | 12.7 | 12.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 15% (w/v) PEG8000, 0.2 M calcium acetate, 0.1 M 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, pH 7.0 |
