6K5H

Structural and catalytic analysis of two diverse uridine phosphorylases in the oomycete Phytophthora capsici.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.98
Spacegroup name	P 2 21 21
Unit cell lengths	66.975, 97.688, 188.865
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.844 - 2.503
R-factor	0.2607
Rwork	0.256
R-free	0.34880
Structure solution method	SAD
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	SOLVE
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	43174	4118
<I/σ(I)>	20.7
Completeness [%]	100.0
Redundancy	13.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		283	0.1 mes monohydrate pH 6.0, 22% PEG 400