6K5G
Structural and catalytic analysis of two diverse uridine phosphorylases in the oomycete Phytophthora capsici
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2018-05-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.246, 153.058, 67.280 |
| Unit cell angles | 90.00, 115.75, 90.00 |
Refinement procedure
| Resolution | 47.509 - 1.574 |
| R-factor | 0.1877 |
| Rwork | 0.186 |
| R-free | 0.21720 |
| Structure solution method | SAD |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.600 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Number of reflections | 144078 | 5837 |
| <I/σ(I)> | 6.6 | |
| Completeness [%] | 98.9 | 98.9 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 283 | 0.1 MES monohydrate PH 6.0, 22% PEG 400 |
