6K5F

Crystal structure of the CLC-ec1 deltaNC in presence of 200 mM NaBr

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	77.36
Detector technology	PIXEL
Collection date	2019-04-02
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92
Spacegroup name	C 1 2 1
Unit cell lengths	230.789, 101.656, 172.003
Unit cell angles	90.00, 132.09, 90.00

Refinement procedure

Resolution	44.700 - 3.203
R-factor	0.2387
Rwork	0.236
R-free	0.29980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ene
RMSD bond length	0.012
RMSD bond angle	1.376
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.700	3.300
High resolution limit [Å]	3.200	3.200
Rmerge	0.094	0.623
Number of reflections	93832	4720
<I/σ(I)>	18.2	1.41
Completeness [%]	99.9	99.8
Redundancy	3.5	3.5
CC(1/2)	0.973	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	PEG 400 25% (v/v), 200mM NaBr 100 mM Tris-SO4