6K4F
SiaC of Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2018-12-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9801 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 35.400, 72.800, 95.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.960 - 1.735 |
| R-factor | 0.1991 |
| Rwork | 0.197 |
| R-free | 0.23480 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.884 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((dev_3386: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.960 | 1.797 |
| High resolution limit [Å] | 1.735 | 1.735 |
| Rmerge | 0.098 | 1.129 |
| Rpim | 0.035 | 0.456 |
| Number of reflections | 13113 | 1176 |
| <I/σ(I)> | 12.05 | |
| Completeness [%] | 98.9 | |
| Redundancy | 8.9 | |
| CC(1/2) | 0.998 | 0.620 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1 M phosphate/citrate, PH4.2, 40% (v/v) PEG 300 |
