6K34
Crystal Structure of DphMB1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97917 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.575, 159.539, 71.132 |
| Unit cell angles | 90.00, 105.09, 90.00 |
Refinement procedure
| Resolution | 39.837 - 2.500 |
| R-factor | 0.1898 |
| Rwork | 0.187 |
| R-free | 0.25260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qz3 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.185 |
| Data reduction software | HKL-2000 |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 47483 | 4674 |
| <I/σ(I)> | 8.8 | |
| Completeness [%] | 98.0 | 96.9 |
| Redundancy | 6.3 | 5.9 |
| CC(1/2) | 0.969 | 0.707 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291.15 | Sodium chloride,Imidazole, Potassium/Sodium tartrate |
