6K2P
Crystal structure of proteinase K from Engyodontium album
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SACLA BEAMLINE BL3 |
| Synchrotron site | SACLA |
| Beamline | BL3 |
| Temperature [K] | 300 |
| Detector technology | CCD |
| Collection date | 2019-01-30 |
| Detector | MPCCD |
| Wavelength(s) | 1.24 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 68.270, 68.270, 108.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.554 - 1.600 |
| R-factor | 0.1626 |
| Rwork | 0.162 |
| R-free | 0.18150 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.015 |
| Data scaling software | CrystFEL |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.600 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 34559 | 1695 |
| <I/σ(I)> | 7.7 | |
| Completeness [%] | 100.0 | |
| Redundancy | 315 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 6.5 | 293 | 0.5 M NaNO3, 0.1 M CaCl2, 0.1 M MES |
