6K2G
Structure of FraE in the monomer state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-19 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.960, 42.890, 71.860 |
Unit cell angles | 90.00, 97.64, 90.00 |
Refinement procedure
Resolution | 35.640 - 2.220 |
R-factor | 0.18927 |
Rwork | 0.187 |
R-free | 0.23023 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vwi |
RMSD bond length | 0.014 |
RMSD bond angle | 1.820 |
Data reduction software | MOSFLM (7.2.2) |
Data scaling software | SCALA (3.3.22) |
Phasing software | PHASER (2.8.2) |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.700 | 2.340 |
High resolution limit [Å] | 2.220 | 2.220 |
Rmerge | 0.162 | 0.594 |
Rpim | 0.078 | 0.277 |
Number of reflections | 16687 | 2412 |
<I/σ(I)> | 7.7 | 2.6 |
Completeness [%] | 99.9 | 100 |
Redundancy | 5.1 | 5.3 |
CC(1/2) | 0.987 | 0.800 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293.15 | 200 mM sodium formate 20% (w/w) PEG 3350 |