6K2C

Extended Hect domain of UBE3C E3 Ligase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	296
Detector technology	PIXEL
Collection date	2018-09-20
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9791
Spacegroup name	P 43 21 2
Unit cell lengths	75.610, 75.610, 220.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.094 - 2.703
R-factor	0.2392
Rwork	0.237
R-free	0.27730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2oni
RMSD bond length	0.008
RMSD bond angle	0.931
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.093	2.796
High resolution limit [Å]	2.700	2.700
Rmeas	0.169
Rpim	0.036	0.106
Number of reflections	18388	1780
<I/σ(I)>	32.45
Completeness [%]	99.9
Redundancy	20.9
CC(1/2)	0.992	0.981

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		296	100mM HEPES 7.5, 20% PEG 10000