6K22

Crystal structure of Ca-bound human Annexin A5 in low salt condition

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	193.15
Detector technology	PIXEL
Collection date	2016-04-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.2325
Spacegroup name	P 63 2 2
Unit cell lengths	92.795, 92.795, 134.055
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.397 - 2.747
R-factor	0.253
Rwork	0.250
R-free	0.30600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xo3
RMSD bond length	0.002
RMSD bond angle	0.411
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.747	2.750
Rmerge	0.145	0.390
Rmeas	0.149	0.415
Rpim	0.032	0.134
Number of reflections	9341	411
<I/σ(I)>	14.8	3.5
Completeness [%]	98.8	87.4
Redundancy	16.8	7.4
CC(1/2)		0.350

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		287	0.2 M Potassium Citrate Tribasic pH 8.3 0.1 M HEPES pH 7.5 17% PEG3350 50uM CaCl2 2mM TCEP