6K1W

Crystal structure of Rhodothermus marinus substrate-binding protein at pH 5.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2018-05-10
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9796
Spacegroup name	P 21 21 21
Unit cell lengths	46.421, 48.613, 58.111
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.290 - 1.500
R-factor	0.1887
Rwork	0.185
R-free	0.21830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5z6v
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.290	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.065	0.349
Rmeas	0.073	0.390
Rpim	0.031	0.170
Number of reflections	21337	1064
<I/σ(I)>	40.125	5.57
Completeness [%]	97.7	99.2
Redundancy	5.4	4.9
CC(1/2)	1.000	0.888

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293.15	Magnesium chloride, BIS-Tris, PEG 3350