6K1G
Crystal structure of the L-fucose isomerase soaked with Mn2+ from Raoultella sp.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-29 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 116.274, 163.278, 196.343 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.960 |
| R-factor | 0.19263 |
| Rwork | 0.189 |
| R-free | 0.25191 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fui |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.320 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0218) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.120 | 0.463 |
| Rmeas | 0.134 | 0.539 |
| Rpim | 0.058 | 0.268 |
| Number of reflections | 73180 | 3296 |
| <I/σ(I)> | 17.343 | 2.293 |
| Completeness [%] | 93.8 | 85.9 |
| Redundancy | 4.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293.15 | HEPES, PEG10000 |
